Time for a quick roundup of some of the highlights from the latest issue of JCB.
Lou et al. reveal that myosin light chain kinase regulates the polarity of migrating zebrafish keratocytes. As explained here, the kinase promotes the accumulation of myosin in lamellipodia, diminishing their size and lifetime so that a single cell can form multiple protrusions.
Milev et al. report that a protein called TRAMM, a subunit of the TRAPP complex that promotes membrane transport at the Golgi complex, also localizes to mitotic chromosomes, where it stabilizes kinetochores and recruits the motor protein CENP-E in order to promote spindle attachment and chromosome congression to the metaphase plate. More here.
And, speaking of moonlighting proteins, Wan et al. reveal that the mitotic proteins BuGZ and Bub3 regulate RNA splicing in interphase, a finding that could provide an alternative explanation for why certain drugs kill cancer cells.
Zhang et al. reveal that the transcription factor FOXO1 delays the healing of diabetic wounds. FOXO1 promotes normal wound healing by enhancing keratinocyte migration, but, as discussed in this week’s In Focus, it slows migration under diabetic, high glucose conditions by stimulating the expression of the peptidase inhibitor SERPINB2 and the chemokine CCL20.
Meanwhile, Nanba et al. describe how tracking the movements of human keratinocytes in culture could help identify epidermal stem cells. Senior author Daisuke Nanba explains his findings and their implications in this month’s biobytes, which also features a special report on the issue of research reproducibility. Daniel Madsen describes the problems associated with identifying the cellular source of matrix proteases in human tumors (Madsen and Bugge), and JCB academic editor Ken Yamada discusses his and Alan Hall’s editorial on how JCB, and the broader cell biology community, can implement a series of best practices to improve research reproducibility. You can listen to the podcast below or subscribe in iTunes.
That’s all for now, but, of course, there are plenty of other interesting papers for you to discover by visiting JCB’s table of contents here.
Cover image of zebrafish keratocytes with varying MLCK expression levels © 2015 Lou et al.